ISO 20743:2013

Determination of antibacterial activity of textile products



Staphylococcus aureus

Escherichia Coli

Klebsiella Pneumoniae



ISO 20743:2013 specifies quantitative test methods to establish the antibacterial activity of all antibacterial textiles including nonwovens.

This ISO standard can be used for registrations of the product.

ISO 20743:2013 is valid to all textile products, including cloth, wadding, thread and material for clothing, bedclothes, home furnishings and miscellaneous goods, regardless of the type of antibacterial agent used (organic, inorganic, natural or man-made) in our case the Si-Quat textile treatment or the method of application (built-in, after-treatment or grafting).

Based on the anticipated application and on the setting in which the textile product is to be used and also on the surface properties of the textile, the user can select the most appropriate of the following three inoculation methods on determination of antibacterial activity:

a) absorption method (an evaluation method in which the test bacterial suspension 

is inoculated directly onto specimens);
b) transfer method (an evaluation method in which test bacteria are placed on an agar plate and transferred onto specimens);
c) printing method (an evaluation method in which test bacteria are placed on a filter and printed onto specimens).
The colony plate count method and the ATP (ATP = Adenosine Tri-phosphate) luminescence method are also specified for measuring the enumeration of bacteria.

Chemical analysis and testing, Cloth, Bacteria, Woven fabrics, Bacteria inhibition tests, Hygiene, Textile fibres, Textile testing, Biological analysis and testing, Textiles, Textile products, Fabric testing, Microbiological-resistance tests, Knitted fabrics, Textile technology, Finishes


One part of the blend is added to 8 parts of neutralizer and 1-part water for 5 minutes to bring the bactericidal activity to a standstill. The final mixture is then attained and incubated for 2 days to allow surviving microorganisms (if any) to proliferate. The bacterial colony is counted and compared against the original culture size.