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EN 1040:2005

Chemical Disinfectants And Antispetics. Quantitative Suspension Test For The Evaluation Of Basic Bactericidal Activity Of Chemical Disinfectants And Antiseptics.



Staphylococcus aureus

Escherichia Coli

Pseudomonas aeruginosa



EN 1040 is a phase 1 suspension test for the evaluation of basic bactericidal activity in chemical disinfectants. The result of a basic suspension test cannot be used to substantiate product claims and it is not valid for product registration. For Si-Quat we have used this standard in the development process of the products and verification of anti-microbial activity on a range of surfaces. The test is applicable to active substances (antibacterial biocides) and to formulations under development that are planned to be used in food, industrial, domestic and institutional, medical and veterinary areas.

Si-Quat has preformed at 10log 5 or above translating to over 99.99% in most cases throughout the development. 


The test standard states the limits to be observed when testing products for basic bactericidal activity. This includes the test microbes, test temperature and contact time. 

Test microbes refer to the obligatory list of microbes that must be used in the test to determine the antimicrobial activity of our Si-Quat product. The obligatory microorganisms are assumed to represent all microbes in its group.

Test temperature refers to the temperature in which the research must be preformed. The general assumption is that disinfectants are less effective in low temperatures compared to higher temperatures.

Contact time refers to the minimum duration a product must remain in contact with the microbes for the product to be effective.

The Test

In a phase 1 suspension test, 8 parts of the test product is added to 1-part test microorganism and 1-part water. The mixture is allowed to interact for the duration of the contact time. 


One part of the blend is added to 8 parts of neutralizer and 1-part water for 5 minutes to bring the bactericidal activity to a standstill. The final mixture is then attained and incubated for 2 days to allow surviving microorganisms (if any) to proliferate. The bacterial colony is counted and compared against the original culture size.

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